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KMID : 0363819930270020270
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Korean Journal of Nuclear Medicine
1993 Volume.27 No. 2 p.270 ~ p.276
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Polymer Formation and Altered Biodistribution of IgG Labeled with 99mTc and Cyclic DTPA
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임상무
우광선/정위섭/오옥두
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Abstract
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Technetium-99m labeling method using bifunctional chelating agent cyclicDTPA has been evaluated with human polyclonal nonspecific IgG. IgG was conjugated with cyclic DTPA with various molar ratio. Reduction of 99mTc was done with Na2S2O4 with
various
molar excess. Labeling efficiency and identification of polymer was confirmed with HPLC using TSK4000 SW column. Polymer was purified with 100 cm Sepharose 6LB column. Cultured 1×10E9 Staphylococcus aureus were injected into rat thigh 24 hours
later
labeled IgG was injected, and in vivo distribution was observed 4 and 24 hours thereafter. Reduction of 99mTc was optimal with the 10000~50000 times molar excess of Na2S2O4. Polymer formation increased with increasing mloar excess of cyclic DTPA
to
IgG.
Three step labeling-labeling DTPA conjugated IgG after reduction of 99mTc-made more polymer than two two step labeling-simultaneous mixing DTPA conjugated IgG, 99mTc and Na2S2O4. 99mTc blood clearance and lower uptake in the abscess and other
organs.
IgG conjugated with 200 times molar excess of cyclic DTPA showed slower blood clearance with 200 times molar excess of cyclic DTPA showed slower blood clearance than that of 200 times molar excess of cyclic DTPA showed slower blood clearance than
that
of 20 times molar excess. In the 99mTc labeling of IgG with cyclic DTPA for the immunoscintigraphy, obtimal labeling condition should be chosen, and effect of the 99mTc labeled IgG polymer should be considered.
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KEYWORD
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